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cd44 apc  (Miltenyi Biotec)


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    Miltenyi Biotec cd44 apc
    Cd44 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd44 apc/product/Miltenyi Biotec
    Average 96 stars, based on 136 article reviews
    cd44 apc - by Bioz Stars, 2026-03
    96/100 stars

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    IL-3 boosts BCSC marker expression and mammosphere formation. ( A , B ) Representative FACS dot plots of ALDH1A1 and <t>CD44/CD24</t> in MDA-MB-436 and ( C , D ) Hs-578T cells upon treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5 ng/ml) and NC (100 pmol) + IL-3 (5 ng/ml) for 24h (n = 3; 3 biological replicates for each n). Data are represented as mean ± SEM. ( E , F ) ALDH activity assay on supernatant of MDA-MB-436 and Hs-578T cell culture upon treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5 ng/ml) and NC (100 pmol) + IL-3 (5 ng/ml) for 24h (n = 3; 3 biological replicates for each n). Data are represented as mean ± SEM. ( G-H ) Mammosphere formation assay of MDA-MB-436 and Hs-578T cells subjected to treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5ng/ml), NC (100 pmol) + IL-3 (5 ng/ml), IL-3Rα blocking mAb (1 ng/ml) + IL-3 (5 ng/ml) and IL-3 blocking mAb (1 ng/ml) + IL-3 (5 ng/ml) (n = 3; 3 biological replicates for each n). Original magnification corresponds to 20X, scale bar: 50 µm. Data are represented as mean ± SEM
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    IL-3 boosts BCSC marker expression and mammosphere formation. ( A , B ) Representative FACS dot plots of ALDH1A1 and CD44/CD24 in MDA-MB-436 and ( C , D ) Hs-578T cells upon treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5 ng/ml) and NC (100 pmol) + IL-3 (5 ng/ml) for 24h (n = 3; 3 biological replicates for each n). Data are represented as mean ± SEM. ( E , F ) ALDH activity assay on supernatant of MDA-MB-436 and Hs-578T cell culture upon treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5 ng/ml) and NC (100 pmol) + IL-3 (5 ng/ml) for 24h (n = 3; 3 biological replicates for each n). Data are represented as mean ± SEM. ( G-H ) Mammosphere formation assay of MDA-MB-436 and Hs-578T cells subjected to treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5ng/ml), NC (100 pmol) + IL-3 (5 ng/ml), IL-3Rα blocking mAb (1 ng/ml) + IL-3 (5 ng/ml) and IL-3 blocking mAb (1 ng/ml) + IL-3 (5 ng/ml) (n = 3; 3 biological replicates for each n). Original magnification corresponds to 20X, scale bar: 50 µm. Data are represented as mean ± SEM

    Journal: Breast Cancer Research : BCR

    Article Title: IL-3/STAT5/miR-155-5p axis supports stem-related pathway reprogramming in TNBC

    doi: 10.1186/s13058-025-02143-1

    Figure Lengend Snippet: IL-3 boosts BCSC marker expression and mammosphere formation. ( A , B ) Representative FACS dot plots of ALDH1A1 and CD44/CD24 in MDA-MB-436 and ( C , D ) Hs-578T cells upon treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5 ng/ml) and NC (100 pmol) + IL-3 (5 ng/ml) for 24h (n = 3; 3 biological replicates for each n). Data are represented as mean ± SEM. ( E , F ) ALDH activity assay on supernatant of MDA-MB-436 and Hs-578T cell culture upon treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5 ng/ml) and NC (100 pmol) + IL-3 (5 ng/ml) for 24h (n = 3; 3 biological replicates for each n). Data are represented as mean ± SEM. ( G-H ) Mammosphere formation assay of MDA-MB-436 and Hs-578T cells subjected to treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5ng/ml), NC (100 pmol) + IL-3 (5 ng/ml), IL-3Rα blocking mAb (1 ng/ml) + IL-3 (5 ng/ml) and IL-3 blocking mAb (1 ng/ml) + IL-3 (5 ng/ml) (n = 3; 3 biological replicates for each n). Original magnification corresponds to 20X, scale bar: 50 µm. Data are represented as mean ± SEM

    Article Snippet: The following anti-human antibodies were used: IL-3Rα-APC, (Milteny Biotec #130-113-322), CD44-FITC (Miteny Biotec #130-113-341), CD24-PE (Milteny Biotec #130-112-656), ALDH1A1-PE (abcam #ab209537).

    Techniques: Marker, Expressing, Saline, Activity Assay, Cell Culture, Tube Formation Assay, Blocking Assay